Interpretting and Plotting SEM Data

Last week, the images of the electrospun fibers was analyzed. From the image, more than 300 fiber diameter lengths were measured using the program ImageJ, as well as pore areas. Using Excel, and the scaling program on ImageJ, this data was converted into either nanometers, or nanometers squared, depending on whether it was a diameter length or a pore area.

For all the fiber diameter lengths, the mean value was calculated to be 108.2 nm, while the standard deviation was 30.5. For the pore areas, the mean value was 8.3 nm2 and the standard deviation was 5.75. Using these measurements and calculations, a table was created for both the diameter lengths and the pore areas and were put into percentiles of 10% differences between values. Using those percentiles, a normal distributive graph was obtained for both, allowing for the spread of the measurements to be illustrated. These tables and graphs, as well as a much more detailed explanation of the results, can be found on the "Data Analysis" page in the top navigation bar

The normal distributive graph for the fiber diameter lengths showed the even spread of the data, explaining the fiber diameter lengths can be within a certain range. The normal distributive graph for the pore areas illustrated that the majority of measurements were closer to the mean value, while there were a few rare large area pore. This showed the discrepancy between the fiber diameter lengths and the pore areas created by them, since the fiber diameter lengths were between a certain range, unlike the random pore areas.